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RF-O2熒光光纖氧氣測量技術——氧氣測量全面解決方案
更新時間：2025-07-01
訪問次數：5352
RF-O2熒光光纖氧氣測量技術是基于REDFLASH光極傳感器技術的氧氣測量技術，由歐洲Pyroscience公司及Graz大學等科學家研制生產，由光極氧氣傳感器、測量儀及軟件組成，廣泛應用于環境科學、生態科學、植物科學、動物科學、海洋科學、生物醫學、生物技術、食品科學等各個領域
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RF-O2熒光光纖氧氣測量技術——氧氣測量全面解決方案
RF-O2熒光光纖氧氣測量技術是基于REDFLASH光極傳感器技術的氧氣測量技術，由歐洲Pyroscience公司及Graz大學等科學家研制生產，由光極氧氣傳感器、測量儀及軟件組成，廣泛應用于環境科學、生態科學、植物科學、動物科學、海洋科學、生物醫學、生物技術、食品科學等各個領域，其主要功能特點如下
- REDFLASH光極氧氣傳感器技術，高精確度、高穩定性、高時空解析度、低能耗、無耗氧、無交叉敏感性
- 傳感器類型靈活多樣，有探頭式、探針式、非接觸式（sensor spot）及納米微粒式等，適應于液體和氣體不同條件下的O2測量
- 有內置sensor spot的流通管和呼吸瓶，非接觸式測量流動液體的溶解氧及呼吸瓶內液體或氣體中氧氣含量
- 輕便緊湊型FireStingO2測量儀，內置水汽、氣壓傳感器，有1、2、4通道供選配，可分別接1個、2個、4個光極氧氣傳感器，另有Mini型FireStingO2-mini供選配
- U盤式PiccolO2測量儀——世界上zui小的O2測量儀，可連接一個O2傳感器，USB口連接電腦，即插即用
測量原理：
REDFLASH光極O2傳感器技術，利用*的O2敏感REDFLASH指示劑，通過610-630nm調制紅光激發，REDFLASH指示劑發出760-790nm紅外熒光，熒光強度隨接觸的O2分子濃度升高而發生熒光淬滅，這種熒光動態通過光纖傳輸到測量儀，測量儀靈敏地檢測其相位漂移并據此換算成O2濃度
應用領域：
- 水體溶解氧測量監測、藻類及藻類生物膜光合作用與呼吸作用測量監測
- 植物光合作用與呼吸作用測量監測
- 水生動物（魚類、水生昆蟲等無脊椎動物、浮游動物等呼吸代謝測量
- 陸生動物、實驗動物、動物組織、血液等呼吸代謝測量
- 土壤、濕地、海洋沉積、河湖沉積剖面O2測量
- 生物反應器、發酵過程、酶動力學、細胞培養等O2測量監測
- 糧食食品儲運、葡萄酒等O2測量監測
- 污水處理、沼氣、垃圾填埋場、有機物降解等O2測量監測
技術指標：
- FireStingO2（FSO2）測量儀：
- 有1通道、2通道、4通道可供選配，分別可接1個、2個和4個O2傳感器，可并聯組成8通道甚至更多通道；另具備一個溫度傳感器通道（可選配4通道溫度傳感器）
- 激發光源620nm，監測器760nm（NIR）
- 采樣頻率：每秒4次
- 內置氣壓傳感器，300－1100mbar，0.06mbar分辨率，精確度±3mbar
- 內置濕度傳感器，0－100%，分辨率0.04%，精確度±0.2%
- 內置溫度傳感器，-40－125°C，分辨率0.01°C，精確度±0.3°C
- 具模擬輸出和自動模式，0－2.5VDC
- USB接口，通過USB口PC供電
- 大小：68x120x30mm，重350g
- PiccolO2 U盤式測量儀：大小僅15x15x54mm，重量約20g，單通道，激發光620nm，檢測器760nm，采樣頻率每秒20次。可并聯組成多通道測量系統。可通過PiccoTHP測量溫濕度和氣壓并進行補償
- 探頭式O2傳感器：直徑3mm，測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui低使用壽命1千萬數據點，存儲時間大于3年（室溫暗處儲放）
- 探針式O2傳感器：有固定探針式、可伸縮探針式、尖頭式及圓頭式等不同類型供選配；探針直徑有50μm、230μm、430μm等規格；測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui快響應時間小于1s（與探針粗細有關），zui低使用壽命1百萬數據點，存儲時間大于3年（室溫暗處儲放）
- 非接觸式（sensor spot）O2傳感器（見下左圖）：用于非接觸性測量監測透明容器中的氧氣含量，傳感器貼用硅膠等貼附在容器內壁，通過固定在外壁的光纖將熒光動態信號傳輸到測量儀以檢測O2濃度；測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui低使用壽命2千萬數據點，存儲時間大于3年（室溫暗處儲放）
- 納米微粒傳感器（參見上右圖）：納米技術，用于非接觸性測量微量液體中O2含量，即時響應，測量范圍0-50%（0-23mg/l），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，存儲時間大于3年（室溫暗處儲放）
- 流通管：內置非接觸式O2傳感器，用于流動液體O2測量監測（如魚類呼吸代謝測量等），測量范圍0-50%（0-23mg/l）（可選配其它范圍），檢測極限0.02%（0.01mg/l），分辨率0.05%（0.025mg/l）@20% O2，精確度±0.2％（0.1mg/l）@20% O2，zui低使用壽命1千萬數據點，存儲時間大于3年（室溫暗處儲放）
- 呼吸瓶：內置非接觸式O2傳感器，用于生物呼吸測量（如藻類、小型魚類、魚卵、昆蟲等），標準配置有4ml和20ml兩種規格
- Pyro Oxygen Logger軟件用于參數設置、校準、數據顯示包括圖表顯示、數據輸出等功能
應用案例：
案例1：法國Bordeaux大學利用FSO2 4通道熒光光纖氧氣測量儀，對Aquitaine海岸沉積樣芯耗氧進行了測量分析，以研究海洋底棲動物活動（bioirrigation）對海岸帶生態系統生態過程及生物地理化學功能（如沉積有機物的再礦化）的影響。
案例2：芬蘭Turku大學利用FSO2和430μm光極氧探針，對南瓜類囊體懸浮液光合放氧進行了測量分析。
案例3：美國Woods Hole海洋學研究所，利用RF-O2非接觸式光極氧氣傳感器（sensor spot），對海洋無脊椎動物呼吸代謝進行了測量分析，以研究其固有的生物鐘與環境脅迫的關系，這些海洋無脊椎動物體重只有0.5－50mg。圖中為翼足類軟體動物在不同濃度CO2條件下的耗氧率。
案例4：澳大利亞海洋科學研究所、瑞典Gothenburg大學等組成的科學小組，利用Pyroscience的REDFLASH氧氣測量技術，對河鱸（Perca fluviatilis）呼吸代謝進行測量分析，以研究其熱耐受性和適應性的生理機制。他們選擇波羅的海核電站附近的一個瀉湖，核電站排出的熱水進入該瀉湖，在過去30年大量魚類因為不適應水溫升高而滅絕，但河鱸卻得以繁盛，該地成為理想的研究氣候變暖對魚類種群影響的“天然實驗室”。他們測量河鱸呼吸代謝率的同時，還測量其靜脈血液在溫度升高狀態下的氧分壓，靜脈血是河鱸心臟供氧的主要來源，高溫條件下靜脈血氧氣含量被認為是其心臟功能的重要限制因子。
案例5：德國Ulm大學利用FSO2測量儀和50μm可伸縮式RFO2探針，對患者腦脊髓液（CSF）樣品溶解氧進行測量分析，以研究探討神經紊亂及神經炎等疾病的生理和診斷。
案例6：德國農業科學與景觀研究機構，利用FSO2測量儀和RFO2探針，對土壤氧氣進行測量，以評估不同種類蚯蚓在低氧條件下對土壤改良的效率。
案例7：西班牙Valladolid大學利用RFO2熒光光纖氧氣測量技術，監測葡萄酒橡木桶O2吸收——對葡萄酒品質至關重要但一直以來缺乏科學的了解。葡萄酒在橡木桶內（3－24個月）的過程溶解氧至關重要，因為O2調節了葡萄酒整個的熟化過程。
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